Why do you have to flame sterilize the inoculating loop until that time and after using?

what is the purpose of heat fixation for the bacterial smear?
Answers:

Im goibg on birth control and im wondering..?

Heat fixation is the procedure used to stick a bacterial smear to a glass slide for viewing by a compound microscope. It denatures the protiens on the surface of the germs causing them to be sticky.
After a smear have been allowed to dry at room warmth, the slide is gipped by tongs or a clothespin and passed through the flame of a Bunsen burner several times to heat-kill and adhere the organism to the slide. Heat fixation cannot be used surrounded by the capsular stain method as heat fixation will shrink or verbs the capsule (glycocalyx) and cannot be see in stains.
Sterlizing of the inoculation loop is essential precise to avoid contamination of the slide preparations and also to prevent contamination of the inoculum plate.

MMR shot? Female problems?

You want to sterilize the innoculating loop before you do a smear because you want to assassinate off any other microbes that might have be on the loop, such as something that landed on it while you be preparing your materials. You only want the germs from your sample to be on the loop.