Why do you have to flame sterilize the inoculating loop since and after using?

what is the purpose of heat fixation for the bacterial smear?
Answers:

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You flame the loop until that time to destroy any organisms already on it to prevent contamination of your culture. You flame it afterward to prevent unintended spread of germs, especially if the bacteria you are culturing is notably virulent (such as O157 E. coli). Heat fixation allows the bacteria to stick to the slide, instead of rinsing past its sell-by date with the an assortment of rinses used during the staining process. I hope this helps!

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Heat fixation is the procedure used to hug a bacterial smear to a glass slide for viewing by a compound microscope. It denatures the protiens on the surface of the microbes causing them to be sticky.
After a smear have been allowed to dry at room heat, the slide is gipped by tongs or a clothespin and passed through the flame of a Bunsen burner several times to heat-kill and adhere the organism to the slide. Heat fixation cannot be used contained by the capsular stain method as heat fixation will shrink or verbs the capsule (glycocalyx) and cannot be see in stains.
Sterlizing of the inoculation loop is essential in a row to avoid contamination of the slide preparations and also to prevent contamination of the inoculum plate.